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KMID : 0613820140240020137
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Journal of Life Science
2014 Volume.24 No. 2 p.137 ~ p.147
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Anti-adipogenic Activity of Cortex ulmi pumilae Extract in 3T3-L1 Preadipocytes
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Jeong Hyun-Young
Jin Soo-Jung
Nam Soo-Wan
Hyun Sook-Kyung
Kim Sung-Gu
Kim Byung-Woo
Kwon Hyun-Ju
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Abstract
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Cortex ulmi pumilae, the cortex of Ulmus davidiana var. japonica, has been used in traditional folk medicine for its anti-inflammatory effect. Although its various bioactivities such as anti-inflammatory, anti-microbial, and anti-cancer, have been reported, the anti-adipogenic activity of cortex ulmi pumilae remains unclarified. In the present study, we investigated the effect of cortex ulmi pumilae extract on adipocyte differentiation in 3T3-L1 preadipocytes. Treatment with cortex ulmi pumilae extract significantly reduced the formation of lipid droplets and triglyceride content in a dose-dependent manner; this is associated with an inhibition of the adipogenic transcription factors, CCAAT/enhancer binding protein α (C/EBPα), C/EBPβ, and peroxisome proliferator-activated receptor r (PPARr). In addition, cortex ulmi pumilae extract treatment during the early stage of adipogenesis showed more efficient anti-adipogenic activity than treatment during other stages of adipogenesis. Cortex ulmi pumilae extract also inhibited cell proliferation and induced G1 arrest of 3T3-L1 cells in the early stage of adipogenesis. This was associated with upregulated expression of Cdk inhibitor p21 and downregulated expression of cyclin E and phospho-Rb, indicating that cortex ulmi pumilae extract blocks mitotic clonal expansion by cell cycle regulation. Taken together, these results suggest that cortex ulmi pumilae extract possesses anti-adipogenic activity through the inhibition of adipocyte differentiation by blocking mitotic clonal expansion.
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KEYWORD
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Adipocyte differentiation, adipogenic transcription factors, anti-adipogenic activity, cortex ulmi pumilae, mitotic clonal expansion
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